Fig. 9

Deleting Pinch1 in chondrocytes and global Pinch2 deletion causes subtle limb shortening and low bone mass in mice. a Whole-body photographs of 2-month-old control and Pinch1^Col2a1; Pinch2^−/− mice. N = 5 mice per group. Scale bar, 1 cm. b, c The femurs, tibiae and humeri of 3-momth-old male control and Pinch1^Col2a1; Pinch2^−/− mice (b). Quantitative data of long bones length (c). *P < 0.05. N = 5 mice per group. Scale bar, 5 mm. d, e H/E staining of tibial sections from 3-month-old male control and Pinch1^Col2a1; Pinch2^−/− mice. Quantification of the data in d (e). The quantitative data were obtained from the areas between the two green dashed lines. N = 5 mice per group. Student’s t test. The results are expressed as the mean ± s.d. *P < 0.05. Scale bar, 20 μm. f 3D reconstruction from μCT scans of the femurs of 3-month-old male control and Pinch1^Col2a1; Pinch2^−/− mice. Scale bar, 500 μm. g–l Quantitative analysis of the BMD, BV/TV, Tb.N, Tb.Sp, Tb.Th, and Ct.Th. N = 5 mice per group. Student’s t test. The results are expressed as the mean ± s.d. *P < 0.05