Fig. 5 | Bone Research

Fig. 5

From: Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation

Fig. 5

PDGFR-β interacts with FAK and TLN1 in ECs. a Strategy for identifying PDGFR-β binding proteins using LC-MS/MS analysis. Bone morrow endothelial cells (BMECs) treated with PDGF-BB (300 ng·mL−1) for 24 h were subjected to immunoprecipitation (IP) with an antibody against PDGFR-β followed by LC-MS/MS analysis. b The PDGFR-β binding proteins FAK and TLN1 were identified with LC-MS/MS analysis. c PDGFR-β was immunoprecipitated from BMECs with an anti-PDGFR-β antibody. The presence of FAK, TLN1, and PDGFR-β in these immunoprecipitates was evaluated with immunoblotting. Western blot analysis of FAK, TLN1, and VEGF in the BMECs treated with different PDGF-BB doses (0-300 ng·mL−1) (d) or for different times at 0 h–36 h (e). Western blot analysis of FAK, TLN1, and VEGF in the BMECs treated with FAK inhibitors (f) or TLN1 siRNA (g)

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