Fig. 4

The expression and function of HOTAIR in BMSCs and osteoblasts. a qRT‒PCR analysis of HOTAIR levels in BMSCs isolated from WT and Prx-HOTAIR TG mice. b qRT‒PCR analysis of Sp7, Runx2, and Alp mRNA levels in BMSCs isolated from WT and Prx-HOTAIR TG mice. c Representative images of ALP staining (top) in BMSCs cultured with osteogenic medium for 3 days and Alizarin red staining (bottom) in BMSCs cultured with osteogenic medium for 21 days. Scale bar, 8 mm. d qRT‒PCR analysis of HOTAIR levels in BMSCs cultured with osteogenic medium for 3 days. e qRT‒PCR analysis of Sp7, Runx2, and Alp mRNA levels in BMSCs cultured with osteogenic medium for 3 days. f Representative images of ALP staining in BMSCs cultured with osteogenic medium for 3 days and Alizarin red staining (below) in BMSCs cultured with osteogenic medium for 21 days. Scale bar, 8 mm. g qRT‒PCR analysis of HOTAIR levels in primary osteoblasts isolated from WT and Bglap-HOTAIR TG mice and cultured with osteogenic medium for 3 days. h qRT‒PCR analysis of Alp, Bglap, and Col1a1 mRNA levels in primary osteoblasts cultured with osteogenic medium for 3 days. i Representative images of ALP staining (top) in primary osteoblasts cultured with osteogenic medium for 3 days. Alizarin red staining (below) in primary osteoblasts cultured with osteogenic medium for 14 days. j qRT‒PCR analysis of HOTAIR levels in primary osteoblasts cultured with osteogenic medium for 3 days. k qRT‒PCR analysis of Alp, Bglap, and Col1a1 mRNA levels in primary osteoblasts cultured with osteogenic medium for 3 days. l Representative images of ALP staining (top) in primary osteoblasts cultured with osteogenic medium for 3 days. Alizarin red staining (below) in primary osteoblasts cultured with osteogenic medium for 14 days. Scale bar, 8 mm. All data are the mean ± s.e.m. from three independent experiments. Two-tailed unpaired Student’s t test was used for statistical evaluations of two group comparisons. *P < 0.05, **P < 0.01, ***P < 0.001