Fig. 7
Osteoblast-specific overexpression of HOTAIR alleviates unloading-induced bone loss. a Representative image showing three-dimensional distal femur trabecular architecture by micro-CT reconstruction from the indicated groups of mice. Representative images of six independent tissues in each group. Ctrl, control group, HU, hindlimb unloading group. Scale bar, 0.5 mm. b Micro-CT measurements for BV/TV, BMD, Tb.N and Tb.Sp at the distal femurs. n = 10 for each group. c Representative images of Ocn staining of the proximal tibia. Scale bar, 100 μm. d ELISA analysis of PINP protein levels in the serum. n = 10 for each group. e qRT-PCR analysis of Alp, Bglap, and Col1a1 mRNA levels in bone tissues. n = 10 for each group. f, g qRT-PCR analysis of HOTAIR and miR-214 levels in bone tissues. n = 10 for each group. h Western blot analysis of Atf4 protein levels in bone tissue. All data are the mean ± s.e.m. Statistical analysis with more than two groups was performed with two-way analysis of variance (ANOVA) with the Å Ãdák post hoc test to determine group differences. All data are the mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001
