Fig. 3: Differential effect of the sequential combination in TNBC and normal cells. | British Journal of Cancer

Fig. 3: Differential effect of the sequential combination in TNBC and normal cells.

From: Sequential targeting of PARP with carboplatin inhibits primary tumour growth and distant metastasis in triple-negative breast cancer

Fig. 3

Impact of the combination of talazoparib and carboplatin using concurrent or sequential approaches on cell proliferation, fork speed, and DNA damage. Using a 9-day (216-hour) treatment assay (a), cell survival was determined for b HCC1806, c MDAMB231, d HCC1143 and e MX1 TNBC cell lines. Using a 72-h treatment assay (a), impact of treatment on fork speed (DNA fibre assay) and DNA damage (flow cytometry) was shown in TNBC cell lines (f–i), and normal cell lines (l–o). f, g, l, m Representative images of DNA fibres with DNA labelling with CldU (magenta) and IdU (green) thymidine analogues. Images were taken at ×63 objective. Scale bars, 10 microns. h, i, n, o Dot plots of fork speed (kb/min), with a mean count of 313 DNA fibres/condition. Line represents median values with interquartile range. Impact of treatments on DNA damage represented by γ-H2AX+ cells in (j, k, p, q) Data are represented as mean +/– SEM. ANOVA with post test for multiple comparisons was performed. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.

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