Fig. 1: Arginine shortage uniquely affects BiP protein and mRNA levels in TNBC cells. | British Journal of Cancer

Fig. 1: Arginine shortage uniquely affects BiP protein and mRNA levels in TNBC cells.

From: Arginine regulates HSPA5/BiP translation through ribosome pausing in triple-negative breast cancer cells

Fig. 1

a A representative Western blot shows BiP abundance in MDA-MB-231 cells grown either in arginine (control, 0 h) or arginine-free medium (R−) for the indicated time periods. Last lane: Arginine (84 mg/L, R rescue) was added to arginine-free medium for an additional 24 h. The relative BiP level (to the 0 h) is shown in italic after normalisation with the GAPDH level. b qRT-PCR analysis of BiP transcript levels in MDA-MB-231 cells under indicated arginine conditions. c, d A representative Western blot shows BiP abundance in MDA-MB-231 cells after treatment with glucose (5 mM, c) or etomoxir (0.5 mM, d) at the indicated time points. The densitometric analysis of a representative BiP as in a. Bars represent mean ± S.D.; *: p < 0.05; **: p < 0.01; (Student’s t-test); n = 3; R: arginine.

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