Fig. 4: Proteomic and pathological characteristics of positive and negative TDLNs.

a Violin plots verifying protein differences in immune cell-associated upregulated genes. b Heatmap of differentially expressed proteins in positive lymph nodes (PosLNs) and negative lymph nodes (NegLNs); c Volcano plot showing differentially expressed proteins in PosLNs and NegLNs; d Dot plot showing the KEGG pathways enriched in the proteins upregulated in NegLNs; e GSEA plot of the T-cell receptor signaling pathway gene set. f Violin plot showing the difference in protein expression between PosLNs and NegLNs in the T-cell receptor signaling pathway gene set. g GSEA plot of the B-cell receptor signaling pathway gene set. h Violin plot showing the difference in protein expression between PosLNs and NegLNs in the B-cell receptor signaling pathway gene set. i, j Immunohistochemical staining of CD3, CD8 and CD19 in PosLNs and NegLNs. i Pathological images. j Violin plot of the quantitative results. PosLN positive lymph node, NegLN negative lymph node, TDLNs tumor-draining lymph nodes, KEGG Kyoto Encyclopedia of Genes and Genomes, GSEA gene set enrichment analysis.