Fig. 4: FBXO22 interacts with RPS5 and reduces its expression through its ubiquitin ligase activity.
From: FBXO22 promotes HCC angiogenesis and metastasis via RPS5/AKT/HIF-1α/VEGF-A signaling axis
A Mass spectrometry identified RPS5 as an interactor of FBXO22. B, C The interaction was confirmed in HEK293T and HCC cell lines. D Confocal immunofluorescence showed that FBXO22 and RPS5 colocalized in the cytoplasm. E Functional regions of FBXO22 and interactions with RPS5 were shown in a schematic diagram. F Constructs were transfected into Hep3B and HepG2 cells. G FBXO22 knockdown cell lines were transfected with the indicated constructs. H The mRNA expression was detected in the indicated cells. Data and error bars are presented as mean ± SD from triplicate independent replicate experiments. The data were analyzed using a paired Student’s t-test for experiments (H). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
