Fig. 4: Aberrant NOTCH and SHH signaling drive CPC in mice. | Cell Death & Differentiation

Fig. 4: Aberrant NOTCH and SHH signaling drive CPC in mice.

From: Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development

Fig. 4

A Wild type, Lcre;NICD1, Lcre;Ptchcko, and Lcre;Ptchcko;NICD1 animals are shown at day E14.5. Notice the cranium defects resulting from enlarged and folded roof plate in the midbrain-hindbrain region of Lcre;Ptchcko and Lcre;Ptchcko;NICD1 animals (white arrowheads). H&E staining and Ki-67 expression are shown of roof plate (upper roof plate marked by red lines) and the CP (black arrows) in the hindbrain in wild type and Lcre;Ptchcko animals, and CPP and abnormal CP growth (black arrowheads) in Lcre;NICD1 and Lcre;Ptchcko;NICD1 animals, respectively. Enlarged roof plate disrupts the cranium in Lcre;Ptchcko and Lcre;Ptchcko;NICD1 animals (red arrows). The upper roof plate is shown in higher magnification in the right (Lcre;Ptchcko;NICD1 animal) and lower (wild type, Lcre;NICD1, Lcre;Ptchcko, and Lcre;Ptchcko;NICD1 animals) panels. Scale bars, 100 µm. Quantification of Ki-67 expression in the upper roof plate and CP in the hindbrain is shown (wild type mice: n = 11; Lcre;NICD1 mice: n = 4; Lcre;Ptchcko mice: n = 3; Lcre;Ptchcko;NICD1 mice: n = 7 for upper roof plate, n = 8 for the CP; mean ± s.e.m., one-way ANOVA, ***P < 0.001; ****P < 0.0001). Data are representative of at least three independent experiments. B Representative results of immunohistochemical staining for OTX2, and AQP1 are shown in the upper roof plate (marked by dotted lines) and the CP (arrows) in the hindbrain at day E14.5 in wild type and Lcre;Ptchcko animals, and CPP and abnormal CP growth (black arrowheads) in Lcre;NICD1 and Lcre;Ptchcko;NICD1 animals, respectively. Residual AQP1-expressing epithelial cells (red arrowhead) are mixed with tumor cells in Lcre;NICD1 animals. Scale bar, 50 µm. Images represent at least three independent experiments.

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