Fig. 2: The regulatory axes of embryonic limb bud development are disturbed by Hnrnpk ablation. | Cell Death & Differentiation

Fig. 2: The regulatory axes of embryonic limb bud development are disturbed by Hnrnpk ablation.

From: Hnrnpk is essential for embryonic limb bud development as a transcription activator and a collaborator of insulator protein Ctcf

Fig. 2

A Representative WISH images showed that Sox9 failed to pattern along with the digits at E11.5 (top) and E12.5 (bottom) in CKO embryos compared to WT. The black boxes indicated the forelimbs, and the red boxes indicated the hindlimbs. Dotted lines indicated the limb buds. The magnification of limb buds was rotated to the same direction. Scale bar: 1 mm. B Representative WISH images showed the expression pattern and decreased signal intensity of Shh at E10.5 (top) and E11.5 (bottom) in CKO embryos compared to WT. The black boxes indicated the forelimbs, and the red boxes indicated the hindlimbs. Dotted lines indicated the limb buds. The arrows indicated the postaxial region of the limb buds. The magnification of limb buds was rotated to the same direction. Scale bar: 1 mm. C Representative WISH images showed vanished signals of Fgf8 at the AER region at E11.5 (top) and E12.5 (bottom) in CKO embryos compared to WT. The black boxes indicated the forelimbs, and the red boxes indicated the hindlimbs. The arrows indicated the AER region. The magnification of limb buds was rotated to the same direction. Scale bar: 1 mm. D Representative WISH images showed decreased signal intensity of Lmx1b at the dorsal region of limb buds at E11.5 (top) and E12.5 (bottom) in CKO embryos compared to WT. The black boxes indicated the forelimbs, and the red boxes indicated the hindlimbs. Dotted lines indicated the limb buds. The magnification of limb buds was rotated to the same direction. Scale bar: 1 mm. E qPCR results confirmed the decreased expression of Sox9, Shh, Fgf8, and Lmx1b at E11.5 in CKO limb buds compared to WT. N = 5 biological replicates. F qPCR results showed that HoxD9-13 were downregulated at E11.5 in CKO limb bud compared to WT. N = 5 biological replicates. The p value was calculated by two-tailed unpaired Student’s t test. Data were shown as mean ± SD. **p < 0.01, ***p < 0.001.

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