Fig. 7: Effect of miR-125b and EVA1A in a tumor xenograft model. | Cell Death & Disease

Fig. 7: Effect of miR-125b and EVA1A in a tumor xenograft model.

From: MicroRNA-125b reverses oxaliplatin resistance in hepatocellular carcinoma by negatively regulating EVA1A mediated autophagy

Fig. 7

HCC cells stably overexpressing miR-125b or EVA1A or 3-MA treatment and treated by oxaliplatin were inoculated subcutaneously into the right flank regions of 4-week-old male BALB/c nude mice. a Representative images of xenograft tumors isolated from nude mice in the different groups. b Tumor size in the different groups. c The expression of EVA1A and miR-125b were detected by western blotting in the indicated groups of tumors. d, e Western blot analysis of autophagy-related proteins and quantified levels of proteins. f, g The TUNEL assay (×400) was performed to determine the apoptotic indices. h, i HCC cells (2 × 106) stably expressing miR-125b or EVA1A were injected into the spleen of BALB/c nude mice under anesthesia. Mice were euthanized 8 weeks after injection, the livers were surgically excised and subjected to H&E staining to detect metastatic liver lesions. Data are expressed as means ± SEM (n = 6). *P < 0.05 compared to pCDH, #P < 0.05 compared to EVA1A

Back to article page