Fig. 6: E-cad participates in the maintenance and activation of PI3K signaling. | Cell Death & Disease

Fig. 6: E-cad participates in the maintenance and activation of PI3K signaling.

From: Oocyte-derived E-cadherin acts as a multiple functional factor maintaining the primordial follicle pool in mice

Fig. 6

a The phosphorylation of both FOXO3a and AKT in the ovaries was affected by E-cad. p-FOXO3a and p-AKT were downregulated in E-cad-KD ovaries, but upregulated in E-cad-OE ovaries, compared with those in the control. b Cellular localization of E-cad and FOXO3a in 5 dpp ovaries. FOXO3a (green) was localized to the nuclei (arrowheads) of the dormant oocytes and translocated to the cytoplasm (arrows) of the activated oocytes in which PI3K signaling was remarkably activated. E-cad (red) expression was increased in the oocytes with cytoplasm-localized FOXO3a. The nuclei were dyed with a Hoechst counter-stain (blue). c Immunofluorescence staining showed that the shuttle of FOXO3a from the nuclei to the cytoplasm in the oocytes was either attenuated by E-cad-KD transfection or enhanced by E-cad-OE transfection compared with the control. The experiments were repeated at least three times, and representative images are shown. Scale bars: 50 μm

Back to article page