Fig. 4: Knockdown of SIRT2 inhibits the activation of the STAT3 signalling pathway in CRC. | Cell Death & Disease

Fig. 4: Knockdown of SIRT2 inhibits the activation of the STAT3 signalling pathway in CRC.

From: Inhibition of SIRT2 limits tumour angiogenesis via inactivation of the STAT3/VEGFA signalling pathway

Fig. 4

a, b The levels of the indicated proteins were measured by using western blot assay in the indicated cells. GAPDH was the internal reference. c, d Western blot assay showing the nuclear total STAT3 and p-STAT3 expression of SW48 and SW480 cells with or without inhibition of SIRT2. e 293T cells were cotransfected with FLAG-STAT3 and HA-SIRT2 plasmids, and then the cell lysate was used to perform an immunoprecipitation assay. Western blot showing the interaction between Flag-STAT3 and HA-SIRT2 in 293T cells. f SW480 cells were lysed and then the cell lysate was used to perform an immunoprecipitation assay. Western blot showing the endogenous interaction between STAT3 and SIRT2 in SW480 cells. g IF staining showing co-localization of SIRT2 (red) and STAT3 (green) in SW480 cells. Scale bar: 50 μm. h IP analyses for IgG and STAT3 in ShNC and ShSIRT2#1 SW480 cells followed by western blot analyses with the indicated antibodies

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