Fig. 8: Expression of linc00665 was upregulated by transcription factor SP1. | Cell Death & Disease

Fig. 8: Expression of linc00665 was upregulated by transcription factor SP1.

From: Long non-coding RNA linc00665 promotes lung adenocarcinoma progression and functions as ceRNA to regulate AKR1B10-ERK signaling by sponging miR-98

Fig. 8

a, b Validation of SP1 siRNA knockdown and overexpression vector efficiency in A549 cells as determined by qRT-PCR and western Blot. c Relative linc00665 expression in A549 cells after transfected with SP1 siRNA or overexpression plasmid. d ChIP assay was used to detect the binding of SP1 protein to linc00665 promoter in A549 and H1299 cells. e, f The sequence –42 to –33-bp upstream of linc00665 was detected in SP1 immunoprecipitates, which was further enhanced upon overexpressing SP1 in A549 and H1299 cells, as determined by ChIP assay. g Expression levels of SP1 mRNA in the 80 paired LUAD tissues by qRT-PCR. h Correlation between SP1 and linc00665 expression in 80 LUAD samples. i Schematic diagram of linc00665-based regulatory mechanism in LUAD cells. NC negative control, NS not significant, LUAD, lung adenocarcinoma, ChIP chromatin immunoprecipitation, qRT-PCR quantitative real-time PCR; **p < 0.01

Back to article page