Fig. 3: BNIP3 promotes keratinocyte migration through regulation of autophagy. | Cell Death & Disease

Fig. 3: BNIP3 promotes keratinocyte migration through regulation of autophagy.

From: RETRACTED ARTICLE: Involvement of autophagy in hypoxia-BNIP3 signaling to promote epidermal keratinocyte migration

Fig. 3

The shBNIP3 stably transfected HaCaT cells and control cells (shNC) were established using lentiviruses. a BNIP3 and LC3 expression levels in the stably transfected HaCaT cells were detected by Western blotting. GAPDH was used as the loading control. b Costaining of LC3 (green) and BNIP3 (red) in HaCaT cells was visualized via confocal microscopy. Nuclei were stained with DAPI (blue). Scale bar = 25 μm. Single-cell motility assays (c, d) and wound-healing assays (e, f) were performed using the indicated cells. Representative images of wound healing, including keratinocyte trajectories. Scale bar = 100 μm. The results of the quantitative analysis are represented by the mean ± SEM (n = 3). *P < 0.05 vs. Norm group, #P < 0.05 vs. the Hypo group

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