Fig. 2: Ectopic expression of miR-129 reduces intermediate neural progenitors.
From: MicroRNA-129 modulates neuronal migration by targeting Fmr1 in the developing mouse cortex
a–n MiR-129 expression plasmids and control plasmids were electroporated into embryonic brain at E14.5, and brain sections of E16.5 were stained with pHH3, Pax6, Tbr2, NeuroD2 and S100 antibodies or using an imaging kit for EdU staining. The scale bar is 500 μm in a and 100 μm in c. o Quantification of GFP+ cells in different regions from different littermates (control: n = 3, miR-129: n = 4). p–s Quantification of GFP+ cells coexpressing the indicated markers from different littermates (control: n = 3, miR-129: n = 4). The results are expressed as the mean ± SD. Comparisons were performed by Student’s t-test, and the statistically significant P-values are shown as *P < 0.05, **P < 0.01 and ***P < 0.001. VZ ventricular zone, SVZ subventricular zone, IZ intermediate zone, CP cortical plate
