Fig. 5: TAZ regulated EGFR signaling pathway via AREG. | Cell Death & Disease

Fig. 5: TAZ regulated EGFR signaling pathway via AREG.

From: TAZ sensitizes EGFR wild-type non-small-cell lung cancer to gefitinib by promoting amphiregulin transcription

Fig. 5

a Gene expression of TAZ and AREG was detected by qPCR and a two-tailed Pearson’s correlation analysis was performed. b Gene expression of AREG in transfected H460 and A549 cells was detected by qPCR. c Protein expression level of TAZ and AREG in transfected H460 and A549 cells was measured by western blotting. GAPDH was used as the loading control. d H460 cells were co-transfected with pGL4.23-AREG-WT + pEX2-TAZ; pGL4.23-AREG-WT + pEX2; pGL4.23-AREG-MUT + pEX2-TAZ; pGL4.23-AREG-MUT + pEX2. Relative luciferase activity was shown in the histogram. e Chromatin immunoprecipitation together with qPCR was performed in shTEAD condition. f Transfected cells were exposed to placebo (PBS) or gefitinib at the concentration of 8 µM. Cells were harvested after 3 h and the protein expression of TAZ, AREG, pAKT, pERK, and pEGFR were measured by western blotting. GAPDH was used as the loading control. Data were shown as mean ± SD. Each experiment was repeated for at least three times. **: p < 0.01

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