Fig. 1: BAALC regulated TNBC cell proliferation, apoptosis, invasion and EMT. | Cell Death & Disease

Fig. 1: BAALC regulated TNBC cell proliferation, apoptosis, invasion and EMT.

From: Long non-coding RNA LRRC75A-AS1 facilitates triple negative breast cancer cell proliferation and invasion via functioning as a ceRNA to modulate BAALC

Fig. 1

a qRT-PCR was carried out to examine the expression level of BAALC in TNBC cells (MDA-MB-231, MDA-MB-468, MDA-MB-436, and HCC-1937) and normal mammary epithelial cells (MCF-10A). b qRT-PCR and western blot verified BAALC knockdown efficiency in sh-BAALC#1/2 transfected MDA-MB-468 and MDA-MB-436 cells. c–d Ki67 immunofluorescence staining (scale bar = 50 μm) and colony formation assays detected the proliferation ability of TNBC cells when down-regulating BAALC. e Mitochondrial membrane potential was measured by JC-1 assay in sh-BAALC#1/2 transfected cells (scale bar = 200 μm). f Flow cytometry analysis evaluated cell apoptosis rate in response to BAALC depletion. g Caspase-3/8/9 activities were measured in sh-BAALC#1/2 transfected cells. h Transwell assay assessed cell invasion before or after knocking down BAALC (scale bar = 200μm). i Western blot detected the expression of EMT-related proteins in TNBC cells transfected with sh-BAALC#1/2. Error bars represent the mean ± SD of at least three independent experiments. **P < 0.01.

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