Fig. 2: MiR-380–3p targeted BAALC and negatively regulated BAALC expression. | Cell Death & Disease

Fig. 2: MiR-380–3p targeted BAALC and negatively regulated BAALC expression.

From: Long non-coding RNA LRRC75A-AS1 facilitates triple negative breast cancer cell proliferation and invasion via functioning as a ceRNA to modulate BAALC

Fig. 2

a RNA pull down assay examined the enrichment of candidate miRNAs in biotin-labeled BAALC group. ba, bb qRT-PCR detected miR-380–3p and miR-450b-5p expression in TNBC cells and MCF-10A cells. c RIP assay detected the enrichment of miR-380–3p, BAALC and miR-450b-5p in anti-Ago2 and anti-IgG groups. da, db qRT-PCR verified the knockdown or overexpression efficiency of miR-380–3p in miR-380–3p-mimics or miR-380–3p-inhibitor transfected cells. e Sequences of wild type/mutant BAALC and miR-380–3p with or without interaction were obtained via starBase prediction (upper); schematic diagram exhibited the interaction between miR-380–3p and BAALC (lower). f Luciferase reporter assay detected the luciferase activity of wild and mutant BAALC under the condition of miR-380–3p knockdown or overexpression. g qRT-PCR and western blot revealed the influence of miR-380–3p downregulation on mRNA and protein expressions of BAALC in TNBC cells. Error bars represent the mean ± SD of at least three independent experiments. **P < 0.01.

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