Fig. 2: Exo-A23187 promotes human umbilical vein endothelial cells (HUVEC) proliferation, tube formation, and migration.
From: STIM1 promotes angiogenesis by reducing exosomal miR-145 in breast cancer MDA-MB-231 cells
A Effect of A23187 on intracellular Ca2+ level in MDA-MB-231 cells was determined with FluoForte Calcium Assay Kit. B Representative electron microscopic image of Exo-A23187. C The markers of exosomes in Exo or Exo-A23187 as determined by western blot. D A23187 increased the production of exosomes in MDA-MB-231 cells. E Images of intercellular trafficking of exosomes by isolated Exo-A23187 labeled with PKH26 in HUVECs. F Tube formation was increased in the Exo-A23187 group compared with the control and Exo group. G Quantification of the tube formation results in (F). H The migration of HUVECs was increased in the Exo-A23187 group compared with the control and Exo group. I Quantification of the HUVEC migration results in (h). Data represent mean ± SEM from three independent experiments (n = 3). *P < 0.05, **P < 0.01.
