Fig. 3: Exo-SKF inhibits human umbilical vein endothelial cells (HUVEC) tube formation and migration through the miR-145 and IRS1 pathways.
From: STIM1 promotes angiogenesis by reducing exosomal miR-145 in breast cancer MDA-MB-231 cells
A Representative time-course recording of intracellular Ca2+ fluorescence showing the inhibitory effect of SKF96365 on Ca2+ influx in MDA-MB-231 cells, which was determined with FluoForte Calcium Assay Kit. B Representative electron microscopic image of Exo-SKF. MDA-MB-231 cells were treated with 10 μM of SKF96365. C The markers of exosomes in Exo and Exo-SKF, as determined by western blot. D SKF96365 did not affect the production of exosomes in MDA-MB-231 cells. E The uptake of Exo-SKF in HUVECs. F The Exo-SKF96365 inhibits tube formation in HUVECs. G Quantification of the results in (F). H Exo-SKF96365 reduces HUVEC migration. I Quantification of the results in (H). J Changes of miRNA level in MDA-MB-231 cells were detected by RT-qPCR after treatment with SKF96365. K The relative levels of miR-145 and miR-449 in Exo-SKF. L The relative levels of miR-145 and miR-449 in HUVECs after Exo or Exo-SKF treatment. M The expression of IRS1 and phosphorylation of c-Raf, ERK, p38, Akt, and mTOR in HUVECs after Exo or Exo-SKF treatment. N Quantification of the results in (M). Data represent mean ± SEM from three independent experiments (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001.
