Fig. 7: FASN knockdown in CAFs or inhibition FA uptake reduce CRC cells migration. | Cell Death & Disease

Fig. 7: FASN knockdown in CAFs or inhibition FA uptake reduce CRC cells migration.

From: Reprogramming of lipid metabolism in cancer-associated fibroblasts potentiates migration of colorectal cancer cells

Fig. 7

a, b Migration of DLD1 cells were analyzed by wound-healing assay a (scale bars, 200 µm) and transwell assay b (scale bars, 100 µm), respectively, after CAFs CM treatment. c Cells migration ability in wound-healing assay and transwell assay was displayed as cell migrated rate (%) and relative permeability, respectively. The values are expressed as the mean ± SD of the average cells number of five randomly selected fields relative to the control group. d Relative mRNA level of metastasis-related genes after co-culturing with CM. The values are expressed as the mean ± SD (n = 3); *p < 0.05; **p < 0.01; ***p < 0.001. e Protein expression of FASN and α-SMA in CAFs after siRNA knockdown; protein expression of Vimentin and E-cadherin in DLD1 after CM treatment. f, g Cells migration ability in transwell assay was displayed as relative permeability. The values shown are expressed as the mean ± SD of the average cells number of five randomly selected fields relative to the control group; *p < 0.05; **p < 0.01; ***p < 0.001.

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