Fig. 4: Effects of hCVPC-EVs on HUVECs migration, tube formation and NRCMs protection.

a, b Representative and quantification of immunohistochemical (IHC) staining for CD31+ endothelial cells (a) and α-SMA+ blood vessels (b) in the border zone of infarcted hearts at day 28 post MI. n = 12 slices from 4 hearts each group. c Tube formation of HUVECs treated with EV-N and EV-H (n = 6). d Migration of HUVECs treated with the EV-N and the EV-H after inhibition of proliferation with 10 μg /mL mitomycin for 2 h (n = 5). Scale bar, 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001.