Fig. 6: ELF3 interacted with SET8 to modulate MARK4 transcriptional activity in HUVECs.
From: High glucose mediates NLRP3 inflammasome activation via upregulation of ELF3 expression
a ELF3 and H4K20me1 were enriched at the MARK4 promoter region. b The putative ELF3 binding site of MARK4. The motif logo and position weight matrix are shown in the upper and lower panels, respectively. c MARK4 promoter activity was determined by luciferase reporter assays. (*P ≤ 0.001, **P ≤ 0.0001, compared with the control group, n = 5/group). d Western blot analysis of SET8, MARK4 and NLRP3 inflammasome expression in control HUVECs, HUVECs overexpressing SET8 and HUVECs overexpressing mutant SET8R259G. e qPCR analysis of SET8, MARK4 and NLRP3 inflammasome expression in control HUVECs, HUVECs overexpressing SET8 and HUVECs overexpressing mutant SET8R259G (*P ≤ 0.001, **P ≤ 0.0001, compared with the control group, n = 5/group). f The effects of ELF3 overexpression or SET8 downregulation on SET8 or ELF3 protein expression in HUVECs. g The effects of ELF3 overexpression on SET8 mRNA expression in HUVECs (*P ≤ 0.001, **P ≤ 0.0001, compared with the control group, n = 5/group). h The effects of SET8 downregulation on ELF3 mRNA expression in HUVECs. (*P ≤ 0.001, **P ≤ 0.0001, compared with the control group, n = 5/group).
