Fig. 3: SNHG12 upregulated the expression of CDCA3 in RCC cells. | Cell Death & Disease

Fig. 3: SNHG12 upregulated the expression of CDCA3 in RCC cells.

From: Long noncoding RNA SNHG12 promotes tumour progression and sunitinib resistance by upregulating CDCA3 in renal cell carcinoma

Fig. 3

a A heat map of correlated genes which were differentially expressed between relatively high SNHG12 expression and relatively low SNHG12 expression according to GSEA analysis based on data from GEO database (GSE53757). b The mRNA expression level of 6 candidate genes in ACHN cells with SNHG12 knockdown or overexpression. c The expression level of CDCA3 was verified by western blot analysis in transfected ACHN and 786-O cells. d Based on TCGA database, the mRNA level of CDCA3 was higher in ccRCC tissues than in normal tissues in TCGA database and higher CDCA3 expression indicated poorer prognosis in RCC. And CDCA3 expression level was positive correlated with SNHG12 expression (r = 0.5055, P < 0.0001). e The high expression of CDCA3 mRNA was related with various clinicopathological factors: G grade, T stage, lymph node metastasis and distant metastases. f Immunohistochemistry (IHC) for CDCA3 in ccRCC tissues and paired normal tissues g The mRNA and protein levels of CDCA3 in five RCC cell lines (786-O, A498, ACHN, CAKI-1, and OSRC-2) and normal cell line (293T). The mRNA and protein levels of CDCA3 in 20 RCC tissues and paired normal tissues. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate mean ± SD.

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