Fig. 2: OIP5-AS1 and ADAMTS8 regulate PTC cell proliferation, migration/invasion and activate EGFR and MEK/ERK pathways in vitro.

A, B OIP5-AS1 and ADAMTS8 expression in four PTC cell lines (TPC-1, BHP5-16, K1, and BCPAP), and in a normal human thyroid epithelial cell line Nthy-ori3-1 were analyzed by qRT-PCR (n = 3). C, D qRT-PCR analysis of ADAMTS8 expression levels in K1 cells and TPC-1 cells, K1 cells were transfected with si-NC/si-OIP5-AS1, while TPC-1 cells were transfected with pcDNA-3.1/OIP5-AS1 (n = 3). E–I K1 cells were co-transfected with OIP5-AS1 and si-ADAMTS8, while TPC-1 cells were co-transfected with si-OIP5-AS1/ADAMTS8 (n = 3). E, F MTT and colony-forming growth assays were performed to determine the proliferation of K1 and TPC-1 cells harboring the different vectors indicated (n = 3). G Transwell assays were performed to determine the migration and invasion capacity of K1 and TPC-1 cells (n = 3). Scale bar = 50 μm. H Wound healing assays were performed to assess the migratory capacity of K1 and TPC-1 cells (n = 3). I Migration and invasion abilities (fold change of migrated or invaded) were calculated, compared with the different vectors in K1 and TPC-1cells. The migratory activity (wound healing) was calculated and compared with those at 0 h (n = 3). J Western blot analysis of phosphorylated EGFR and total EGFR in TPC-1 and K1 cells (n = 3). K Phosphorylated and total Akt, ERK, and MEK levels measured by western blot analysis in TPC-1 and K1 cells (n = 3).*p < 0.05, **p < 0.01, and ***p < 0.001.