Fig. 3: CircUBE2Q2 promotes GC development by serving as a miRNA sponge of miR-370-3p. | Cell Death & Disease

Fig. 3: CircUBE2Q2 promotes GC development by serving as a miRNA sponge of miR-370-3p.

From: Circular RNA UBE2Q2 promotes malignant progression of gastric cancer by regulating signal transducer and activator of transcription 3-mediated autophagy and glycolysis

Fig. 3

A Venn diagram showing the overlap of potential target miRNAs of circUBE2Q2 predicted by miRanda and PITA. B Transfection efficiency of circUBE2Q2 probe in MKN-45 and BGC-823 cell lines. C Ten potential target miRNAs were pulled down and confirmed by qRT-PCR in MKN-45 cell line. D Dual-luciferase reporter assay used to detect the relative luciferase activity in MKN-45 cells cotransfected with luc-circUBE2Q2 and miR-370-3p mimics. E FISH for circUBE2Q2 and miR-370-3p in MKN-45 and BGC-823 cell lines, scale bar = 10 µm. F Expression level of miR-370-3p in GC tissues relative to matched normal tissues via qRT-PCR (n = 60). G CCK-8 analysis of the cell proliferation ability in MKN-45 cells cotransfected with circUBE2Q2-specific siRNA or miR-370-3p inhibitors. H Colony formation assay of the cell proliferation ability in cotransfected MKN-45 cells. I EdU analysis of the cell proliferation ability in treated MKN-45 cells. J Transwell invasion and migration assay in treated MKN-45 cells. K The growth of gastric cancer organoid models cotransfected with circUBE2Q2-specific siRNA or miR-370-3p inhibitors, scale bar = 25 µm. L Western blot analysis of EMT-related genes (TWIST, Snail, Slug, N-cadherin and E-cadherin) with proteins treated with circUBE2Q2-specific siRNA or miR-370-3p inhibitors in MKN-45 cell line. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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