Fig. 6: ANTPTL8 knockout enhanced hypertrophic and fibrotic features in the heart at 4 weeks after TAC.
From: ANGPTL8 is a negative regulator in pathological cardiac hypertrophy
Representative images of H&E A (scale bar, 500 μm (up) and 75 μm (down), respectively) and WGA staining B (scale bar, 500 μm and 75 μm, respectively) in the indicated groups (n = 5 mice in each group). C Picrosirius red-stained transverse sections of the left ventricles from the indicated groups (scale bar, 500 μm, n = 6 mice in each group). D Representative immunohistochemical images for collagen I in mouse tissue with sham and TAC surgery in the indicated groups (scale bar, 500 μm and 75 μm, respectively, n = 6 mice in each group). Quantification of the myocyte-sectional area (E), myocardial interstitial fibrosis (F), myocardial vessel fibrosis (G) and collagen I-positive area (H) of slices from WT and ANGPTL8-KO mice in the indicated groups (n = 5 mice in each group). I, J qRT–PCR was performed to determine the mRNA levels of ANF and BNP in the indicated groups (n = 4 mice in each group). Representative Western blots (K) and quantification (L) of the protein levels of BNP in cardiac tissue from WT and ANGPTL8-KO mice in the indicated groups (n = 4 mice in each group), *P < 0.05, **P < 0.01, ***P < 0.001.
