Fig. 7: ALYREF-RABL6-TK1 m5C related axis predicts poorest overall survival in UCB.
A Pearson correlation analysis showing the association between RABL6, TK1 and ALYREF mRNA expression in the SYSUCC cohort. n = 36. Shaded regions showed the 95% confidence interval. B Pearson correlation analysis showing the association between RABL6, TK1 and ALYREF mRNA expression in TCGA cohort. n = 430. Shaded regions showed the 95% confidence interval. C Representative IHC staining and double immunofluorescence staining images of ALYREF, RABL6 and TK1 in two UCB tissues with high (the first row) or low expression (the second row). Blue (DAPI) = cell nuclei, red (Cyanine 3) = ALYREF, green (Alexa 488) = RABL6/TK1. Scale bars, 100 μm. D Kaplan–Meier analysis of data of 170 UCB patients from SYSUCC showing the correlation between different expression patterns of ALYREF/ RABL6 (Top, P <0.001) and OS, the correlation between different expression patterns of ALYRREF/TK1 (Medium, P <0.001) and OS, and the correlation between different expression patterns of ALYREF/ RABL6/ TK1 (Bottom, P <0.001), and OS. The P-values were calculated by a log-rank test. E Schematic illustration showing that m5C dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating mRNAs splicing and stabilization.
