Fig. 3: RNF157-AS1 interacted with HMGA1 and EZH2 in the nucleus. | Cell Death & Disease

Fig. 3: RNF157-AS1 interacted with HMGA1 and EZH2 in the nucleus.

From: Differential effects of the LncRNA RNF157-AS1 on epithelial ovarian cancer cells through suppression of DIRAS3- and ULK1-mediated autophagy

Fig. 3

A Subcellular localization analysis suggested that RNF157-AS1 was localized mainly in the nucleus in EOC cells. B The RPISeq database showed that RNF157-AS1 had a very high interaction predicting score with HMGA1 protein (RF:0.8; SVM:0.88). C RNF157-AS1 expression had a positive correlation with HMGA1 expression (R = 0.32, P = 2.5*10−13) according to the GEPIA database. D Western blot analysis showed that RNF157-AS1 pulled down a greater amount of protein than did the control. E The RIP assay suggested HMGA1 interacted with RNF157-AS1 in not only RNF157-AS1 overexpression but also control EOC cells. F According to the RPISeq database, EZH2 had the highest interaction predicting score with RNF157-AS1 among the four subunits of the PRC2 complex. G EZH2 had a similar interaction predicting scores between RNF157-AS1 and HOTAIR; H RNF157-AS1 had a positive correlation with HMGA1 (R = 0.25, P = 5.6*10−9) according to the GEPIA database. I Western blot analysis showed that EZH2 was present only in the RNF157-AS1 (sense) precipitate compared with the control (antisense) precipitate. J The RIP assay suggested EZH2 interacted with RNF157-AS1 in not only RNF157-AS1 overexpressing but also control EOC cells. Data were obtained from at least three independent experiments. ***P < 0.001 (t-test).

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