Fig. 2: Overexpression of DAPL1 in the RPE of Dapl1−/− mice inhibits EMT in vivo. | Cell Death & Disease

Fig. 2: Overexpression of DAPL1 in the RPE of Dapl1−/− mice inhibits EMT in vivo.

From: DAPL1 prevents epithelial–mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy

Fig. 2

A At 2 weeks after infection with AAV9-NC or AAV9-DAPL1, western blotting showed the protein expression levels of HA tag, DAPL1, ZO-1, E-cadherin, ZEB1, N-cadherin, vimentin, and SNAIL in RPE cells of Dapl1−/− mice. B The bar graph shows quantification of the bands based on the results in (A). N = 3. C At 2 weeks after infection with AAV9 or AAV9-DAPL1, immunostaining images of ZO-1 in RPE flat mounts of 6-week-old Dapl1−/− mice. D At 2 weeks after the injection of AAV9-NC or AAV9-DAPL1, with the subsequent subretinal injection of sodium hyaluronate to cause retinal detachment for 10 days, histological images of H&E staining show retinal structures from 6-week-old Dapl1−/− mice. Note that subretinal membranes were observed in the control AAV9-NC- but not in the AAV9-DAPL1-injected eyes (black arrows). E The bar graph shows quantification of the area of the subretinal membrane based on the results from (D). F Immunohistochemistry for OTX2 (white arrow indicated) on the surfaces of the detached retinas of Dapl1−/− mice after the injection of AAV9-NC or AAV9-DAPL1 for 2 weeks. G Immunohistochemistry images showing α-SMA expression (white arrow indicated) on the surfaces of the detached retinas of Dapl1−/− mice after the injection of AAV9 or AAV9-DAPL1 for 2 weeks. N = 6, ** indicates P < 0.01. Scale bar: 50 μm.

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