Fig. 5: Deletion of p53 in Osx-Mdm2^fl/+ mice results in osteosclerosis.

A Direct fluorescence image of a bone section derived from an 8-week-old Osx-Mdm2^fl/+;mTmG mouse showing the population of MSCs as GFP+ as well as trabecular bone formation (white arrow). The red box area is magnified in the adjacent panel. B Direct fluorescence image of a bone section derived from a 8 weeks-old Osx-Cre;Trp^fl/fl;Mdm2^fl/+;mTmG mouse showing the population of MSCs as well as trabecular bone formation (white arrows). The red box area is magnified in the adjacent panel. C Micro-CT scan of bones in the indicated mice at 6 months of age showing the trabecular bone formation inside the BM cavity. D Bar graph showing the quantification of trabecular bone density in the indicated mice (n = 3; ***p < 0.001, Student’s t test). E Histomorphometry of bones isolated from indicated mice showing the density of trabecular (yellow) and cortical bone (white) at the level marked by red dash line. F Microscopic view of H&E staining of bones derived from the indicated mice. The black box is magnified in the adjacent image. The black arrows mark the hematopoietic cells trapped inside trabecular bone. G Representative image of reticulin staining of bones derived from the indicated mice. The black arrows mark the reticulin positive cells.