Fig. 2: Severe renal injury in HIF-1α CTAD−/− mice with UUO. | Cell Death & Disease

Fig. 2: Severe renal injury in HIF-1α CTAD−/− mice with UUO.

From: Activation of HIF-1α C-terminal transactivation ___domain protects against hypoxia-induced kidney injury through hexokinase 2-mediated mitophagy

Fig. 2

a qRT-PCR analysis of KIM-1 expression. The relative levels were normalized to β-actin (n = 6). b Representative images of KIM-1 expression in kidney tissues from UUO mice were assessed by immunohistochemistry (n = 6). Scale bars, 50 µm. c Histological changes (PAS staining) on day 3 after UUO (left, n = 6). Scale bar, 100 µm. The quantification of tubular injury based on PAS staining (right, n = 6). d mRNA expression of inflammatory factors (MCP-1, TNF-α, and IL-1β) in the UUO kidney assessed by qRT-PCR. The relative levels were normalized to β-actin (n = 6). e Representative F4/80 stained kidney sections from UUO mice (left). Scale bars, 50 µm. The semiquantitative population of F4/80+ macrophages infiltrating the UUO kidney (right). Data are shown as mean ± SEM of 6 mice. **p-value < 0.01 versus the WT mice (t-test).

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