Fig. 7: UHRF1 and GLI1 are upregulated in HCC tissues and correlated with poor prognosis. | Cell Death & Disease

Fig. 7: UHRF1 and GLI1 are upregulated in HCC tissues and correlated with poor prognosis.

From: UHRF1 inhibition epigenetically reprograms cancer stem cells to suppress the tumorigenic phenotype of hepatocellular carcinoma

Fig. 7

A The expression of UHRF1 was analyzed by RT-PCR in our in-house HCC cohort 1 (n = 75) (paired Student’s t test) (left). UHRF1 mRNA expression in patients with different TNM stages (unpaired Student’s t test) (right). B Immunohistochemistry staining of UHRF1 in cohort 2 HCC tissue microarray (n = 177). Enlarged images are shown in the right panel. C IHC scores of UHRF1 protein expression in patients with different neoplasm histological grades in the clinical cohort 2 HCC tissue microarray. D Kaplan–Meier overall survival curves of cohort 2 HCC patients with normal or overexpressed UHRF1 (IHC score >50). E The mRNA expression of GLI1 positively correlated with UHRF1 (TCGA-LIHC dataset). F Detection and quantification of UHRF1, GLI1, CD44, and CD133 expression in HCC tumor (T) tissues and their matched nontumor (NT) tissues by WB analysis. G Schematic diagram showing that UHRF1 inhibition epigenetically reprograms CSCs to suppress tumorigenesis and CSC features through GLI1/Hedgehog and Wnt signaling in liver cancer. This figure was created with BioRender software (biorender.com). Mean ± SD. ∗P < 0.05, ∗∗∗∗P < 0.0001.

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