Fig. 6: LINC00839 promotes c-Src-mediated β-catenin phosphorylation.
A The enrichment of c-Src in pull-down products of LINC00839 was measured by western blot. B RNA immunoprecipitation with an anti-c-Src antibody was used to assess whether c-Src binding to LINC00839 in GSCs. C GSCs were transfected with or without RNase If. Immunoprecipitation with anti-β-catenin and anti-c-Src antibodies were performed. D GSCs were transfected with or without RNase If. IF assays were performed to analyze the co-localization of β-catenin (Red) and c-Src (Green). E GSCs were transfected with control or LINC00839 ASO. Immunoprecipitation with an anti-β-catenin antibody was performed. F Control or METTL3 deleted GSCs were co-transfected with LINC00839. Immunoprecipitation with an anti-β-catenin antibody was performed. G Control or YTHDF2 deleted GSCs were co-transfected with LINC00839. Immunoprecipitation with an anti-β-catenin antibody was performed. H. GSCs were transfected with FL or TL LINC00839. Immunoprecipitation with an anti-β-catenin antibody was performed. I In vitro β-catenin phosphorylation assay using recombinant β-catenin, c-Src proteins, and in vitro-transcribed RNA transcripts as indicated in NETN buffer with the presence of 500 µM ATP. Immunoblots were used to detect β-catenin phosphorylation level. J Luciferase activity (TOP/FOP) in control or LINC00839 overexpressing MES28 co-transfected with WT or Y654E mutation β-catenin. **p < 0.001. K Control or LINC00839 overexpressing GSCs were co-transfected with WT or Y654E mutation β-catenin. Representative images of spheres were photographed. Scale bars, 1 mm. L, M Control or LINC00839 overexpressing GSCs were co-transfected with WT or Y654E mutation β-catenin. The apoptotic rates were measured by flow cytometry. **p < 0.001.
