Fig. 3: Deficiency of BAP1 inhibits NB cell growth in vitro and in vivo through MYCN.

A Immunoblot (IB) analyses of whole-cell lysate (WCL) from shBAP1 BE2C cells stably expressing MYCN. B Growth curves of BE2C cells with shRNA-mediated BAP1 knockdown (shBAP1) and stably expressing MYCN. C Colony-formation assay. Depletion of endogenous BAP1 in BE2C cells displays moderate decrease in colony-formation ability and overexpression of MYCN will partly recover the colony-formation ability. The number of colonies were counted and quantified. Data are shown as mean ± SD for three independent experiments. P value was indicated in figure, t test. BE2C cells were infected with indicated lentiviral shRNAs and retroviral MYCN. D Representative images for the wound-healing assays of BE2C cells with BAP1 knockdown and MYCN overexpression. The wound edges are indicated by yellow lines. Scale bar, 100 μm. E The quantitative results of (D) (n = 8). The y axis represents the relative wound area. Error bars represent s.d. from eight repeats. t test. F Representative images (n = 5) of migrated BE2C cells infected with the indicated lentiviral shBAP1 and/or stable overexpression of MYCN. Scale bar, 100 μm. G Quantification of the migrated cells in (F). In all plots, data are shown as mean ± s.d. for five independent experiments. H In vivo tumor growth was monitored at the indicated time points. I The weights of the dissected tumors in Supplementary Fig. 3C. J The body weights of the tumor-bearing mice were measured at the indicated time points. K In vivo orthotopic tumor growth was monitored over the indicated time period by detecting and analyzing the bioluminescence signals. L The weight of kidney + adrenal gland and kidney + tumor in Supplementary Fig. 3E (n = 5).