Fig. 4: UUO-induced kidney fibrosis is ameliorated in heterozygous Plk1 knockout mice.
From: Plk1 promotes renal tubulointerstitial fibrosis by targeting autophagy/lysosome axis
A, B Representative images of HE and Masson staining and immunohistochemistry detection of FN, F4/80 in obstructive or contralateral kidneys of PWT and PKO + /− mice and quantification. Scale bar = 50 µm. C, D Western blot analysis of FN, α-SMA, Collagen III, LC3, Plk1 and P62 in obstructed or contralateral kidneys of PWT and PKO + /− mice, and densitometry analysis of the bands. The GAPDH for Collagen III in this blot was from same experiment, same samples and processed in parallel. E qRT-PCR analysis of FN, α-SMA, Collagen I and Collagen III in obstructed or contralateral kidneys of WT and PKO + /− mice. n = 3–6. *P < 0.05, **P < 0.01, ***P < 0.001.
