Fig. 6: JMJD6 demethylates BRD4 to inhibit BRD4 phosphorylation.
From: Methylation of BRD4 by PRMT1 regulates BRD4 phosphorylation and promotes ovarian cancer invasion
a IB analysis of WCL and IP of JMJD6 interaction of exogenous BRD4 in 293T cells transfected with HA-BRD4-470 and GST-JMJD6. b–d IB analysis of WCL and IP analysis of JMJD6 interaction of endogenous BRD4 in 293T cells, A2780 cells, and SKOV3 cells. e IB analysis of WCL and IP derived from HEK293T cells transfected with HA-BRD4-470, Flag-PRMT1, and GST-JMJD6. f IB analysis of WCL and IP derived from HEK293T cells transfected with HA-BRD4-470, Flag-PRMT1, GST-JMJD6-WT, or GST-JMJD6-H187A/D189A. g IB analysis of ADMA and E-cadherin derived from HEK293T cells transfected with HA-BRD4-470, Flag-PRMT1, and GST-JMJD6. h and i IB analysis of BRD4-S492/494 derived from A2780 cells and SKOV3 cells infected with shJMJD6. j and k The interaction of up-regulated genes in sgPRMT1 and shBRD4 (j). The interaction of down-regulated genes in sgPRMT1 and shBRD4 (k).
