Fig. 6: BEX2 promotes tumor growth in vivo. | Cell Death & Disease

Fig. 6: BEX2 promotes tumor growth in vivo.

From: Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells

Fig. 6

A–C BALB/c nude mice were injected subcutaneously with stably transduced H1299 cells. The transplanted tumors were removed and photographed (A). Tumors were isolated, and the weight (B) and growth (C) were measured. D Representative immunohistochemical staining for Ki67 and cleaved CASP3 in tumor tissues obtained from each experimental group. Dot plots show the mean value for the percentage of Ki67 and cleaved CASP3-positive cells with statistical evaluation (n = 4). Scale bars: 50 μm. E Schematic showing the model of xenografts. A549NC, A549BEX2 or A549BEX2K59R cells were injected subcutaneously into the BALB/c nude mice (n = 5 per group). After tumor inoculation for 6 days, doxorubicin (3 mg/kg) was intraperitoneally injected at intervals of 4 days. The control groups were injected with saline. After 21 days, the mice were then euthanized. F–H The transplanted tumors were removed and photographed (F). Tumors were isolated, and the weight (G) and growth (H) were measured. I Immunohistochemistry analysis showing Ki67, cleaved CASP3 and BEX2 expression in the indicated tumor tissues. Scale bar: 50 μm. Data are presented as the mean ± SEM, and statistical significance was assessed by two-way ANOVA (B–D, G, H), *P < 0.05, **P < 0.01, ***P < 0.001.

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