Fig. 3: miR-672-5p was transferred from CECs to microglia. | Cell Death & Disease

Fig. 3: miR-672-5p was transferred from CECs to microglia.

From: Cerebral endothelial cell-derived extracellular vesicles regulate microglial polarization and promote autophagy via delivery of miR-672-5p

Fig. 3

A The schematic diagram of microglia sequencing analysis after CEC-EVs treatment. B The heat map plot of the upregulated and downregulated miRNAs between microglia treated with CEC-EVs and PBS. C Validation of upregulated genes by qRT-PCR. Unpaired Student’s t test. D qRT-PCR was used to explore the expression of miRNAs including miR-128-3p, miR-672-5p, miR-3058-5p, miR-574-3p and miR-762 in activated microglia treated with EVs. Unpaired Student’s t test. E The EVs secretion inhibitor, GW4869, inhibited miR-672-5p transfer from CECs to microglia. one-way ANOVA. F Inhibition effect of GW4869 on miR-672-5p transfer was determined by qRT-PCR. One-way ANOVA. G miR-672-5p knockdown in CECs and the knockdown efficiency was confirmed by qRT-PCR, one-way ANOVA. Effect of miR-672-5p knockdown in CECs on miR-672-5p expression in CEC-EVs (H) and microglia (I), respectively, one-way ANOVA. All data are present as means ± SD (n = 3). ns not significant, **p < 0.01 compared to control group. ##p < 0.01 compared to miR-NCKD-CEC or miR-NCKD-EVs group.

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