Fig. 7: Twist1 directly interacts with both PPARĪ³ and RXRĪ± in 3T3-L1 cells.
From: Deciphering the interaction between Twist1 and PPARĪ³ during adipocyte differentiation
A Non-denaturing electrophoresis to detect the binding between Twist1 and PPARĪ³. B Expressions of Twist1 and PPARĪ³ detected by WB at day 8 of differentiation. C Direct interaction between Twist1 and PPARĪ³ assessed by co-IP assay. Twist1-OE 3T3-L1 cells were transfected with pcDNA3.1(+)-PPARĪ³-ECFP plasmids. Cells were subsequently lysed and immunoprecipitated (IP) with anti-Flag antibody and immunoblotted (IB) with anti-GFP or anti-Twist1 antibody or IP with anti-PPARĪ³ antibody and IB with anti-Twist1 or anti-PPARĪ³ antibody. D Direct interaction between Twist1 and RXRĪ± examined by co-IP assay. Twist1-OE 3T3-L1 cells were transfected with pcDNA3.1(+)-RXRĪ±-EYFP plasmids. Cells were subsequently lysed and IP with anti-Flag antibody and IB with anti-GFP or anti-Flag antibody or IP with anti-GFP antibody and IB with anti-Twist1 or anti-GFP antibody.
