Fig. 2: FAM83F promoted cell proliferation, inhibited apoptosis, and accelerated cell cycle progression in CC cells. | Cell Death & Disease

Fig. 2: FAM83F promoted cell proliferation, inhibited apoptosis, and accelerated cell cycle progression in CC cells.

From: Upregulation of FAM83F by c-Myc promotes cervical cancer growth and aerobic glycolysis via Wnt/β-catenin signaling activation

Fig. 2

A, B FAM83F was stably silenced in SiHa cells by transfection and selection. FAM83F expression was confirmed using qPCR and western blotting analysis. C CCK-8 assays indicated that knockdown of FAM83F inhibited the cell proliferation ability of SiHa cells. D The effect of FAM83F knockdown on cell apoptosis was determined in SiHa cells using flow cytometry. E Cell cycle distribution was determined in SiHa cells using flow cytometry analysis after FAM83F knockdown. The percentage of cells in the G0/G1, S, and G2/M phases was calculated. F, G FAM83F was overexpressed in HeLa cells by transfection and selection. FAM83F expression was confirmed using qPCR and western blotting analysis. H CCK-8 assays indicated that overexpression of FAM83F promoted the cell proliferation ability of HeLa cells. I The effect of FAM83F overexpression on cell apoptosis was determined in HeLa cells using flow cytometry. J Cell cycle distribution was determined in HeLa cells using flow cytometry analysis after FAM83F overexpression. The percentage of cells in the G0/G1, S, and G2/M phases was calculated. The data represent the mean ± SD of three independent experiments, and the level of significance was indicated by ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. ns no significance (p > 0.05).

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