Fig. 6: Propagation of inflammatory cytokines after CMI initiates trained immunity in photothrombotic stroke cortex.
From: Cortical microinfarcts potentiate recurrent ischemic injury through NLRP3-dependent trained immunity
A Schematic diagram of experimental design. Quantitative ELISA analysis of TNF-α (B), IL-6 (C), IL-1β (D), and IL-10 (E) expression in ipsilateral cortex, contralateral cortex, and peripheral serum after PT stroke, n = 12 mice per group. One-way ANOVA was used to analyze the data. For TNF-α analysis, ipsilateral cytokines: F4, 55 = 70.40, p < 0.0001; Sham vs. CMI 6h: p < 0.0001; Contralateral cytokines: F4, 55 = 147.1, p < 0.0001; Sham vs. CMI 6h: p < 0.0001. Serum cytokines: F4, 55 = 2.047, p = 0.1004; Sham vs. CMI 3h: p = 0.6059 (B). For IL-6 analysis, ipsilateral cytokines: F4, 55 = 56.01, p < 0.0001; Sham vs. CMI 6h: p < 0.0001. Contralateral cytokines: F4, 55 = 23.23, p < 0.0001; Sham vs. CMI 6h: p < 0.0001. Serum cytokines: F4, 55 = 4.546, p = 0.0030; Sham vs. CMI 6h: p = 0.7525 (C). For IL-1βanalysis, ipsilateral cytokines: F4, 55 = 7.952, p < 0.0001; Sham vs. CMI 6h: p = 0.0004. Contralateral cytokines: F4, 55 = 34.73, p < 0.0001; Sham vs. CMI 6h: p < 0.0001. Serum cytokines: F4, 55 = 3.635, p = 0.0107; Sham vs. CMI 6h: p = 0.0207 (D). For IL-10 analysis, ipsilateral cytokines: F4, 55 = 35.99, p < 0.001; Sham vs. CMI 6h: p < 0.0001. Contralateral cytokines: F4, 55 = 11.86, p < 0.0001; Sham vs. CMI 6h: p < 0.0001. Serum cytokines: F4, 55 = 5.919, p = 0.0005; Sham vs. CMI 6h: p = 0.0047 (E) with Tukey’s correction. F Schematic diagram of experimental design. Quantitative ELISA analysis of TNF-α (G), IL-6 (H), IL-1β (I), and IL-10 (J) expression in ipsilateral cortex, contralateral cortex, and peripheral serum after PT stroke in WT and \({{NLRP}3}_{{cxc}3r1}^{-/-}\) mice. n = 12 mice per group. Two-way ANOVA for analysis. Ipsilateral cytokines: for interaction: p = 0.0166. So t-tests was used to detect the difference between groups. CMI vs CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.0233. Contralateral cytokines: for interaction: p = 0.0489. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p < 0.0001. Serum cytokine: for interaction: p = 0.2285. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.8071 (G); For IL-6 analysis, ipsilateral cytokines: for interaction: p = 0.0052. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.0100. Contralateral cytokines: for interaction: p = 0.0544. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.0317. Serum cytokine: for interaction: p = 0.1273. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.3334 (H); For IL-1βanalysis, ipsilateral cytokines: for interaction: p = 0.0040. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.0031. Contralateral cytokines: for interaction: p < 0.0001. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p < 0.0001. Serum cytokine: for interaction: p = 0.0774. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.6185 (I); For IL-10 analysis, ipsilateral cytokines: for interaction: p = 0.9477. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.9222. Contralateral cytokines: for interaction: p = 0.6117. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.9997. Serum cytokine: for interaction: p = 0.3239. CMI vs. CMI+\({{NLRP}3}_{{cxc}3r1}^{-/-}\): p = 0.9192 (J) with Turkey’s corrections. Data are presented as mean ± standard deviation (SD), *p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.
