Fig. 3: CBX4 suppresses the invasion and metastasis of LUAD cells in vitro and in vivo.
From: CBX4 plays a bidirectional role in transcriptional regulation and lung adenocarcinoma progression
A A549 and H1299 cells expressing control or CBX4 shRNA were subjected to wound-healing assays. B Transwell assays were performed in A549 and H1299 cells expressing control or CBX4 shRNA. Scale bars, 100 μm. C Wound-healing assays were performed using cells overexpressing empty vectors or Flag-CBX4. D Transwell assays were carried out using A549 and H1299 cells with or without CBX4 overexpression. Scale bars, 100 μm. For figures A–D, each bar represents the mean ± SD for n = 3; ns: no significant, *P < 0.05, **P < 0.01, ***P < 0.001, indicating shCBX4 versus control, Flag-CBX4 versus vector (Student’s t-test). E A549 cells stably expressing control shRNAs, CBX4 shRNAs, empty vector, or Flag-CBX4 were intravenously injected into the tail of female athymic nude mice. The mice were decapitated 8 weeks later, and the number of pulmonary metastatic nodules was counted, highlighted by arrows. Each bar represents the mean ± SD for 7 animal measurements; *P < 0.05, **P < 0.01, ***P < 0.001, indicating shCBX4-1 versus control, Flag-CBX4 versus vector (Student’s t-test).
