Fig. 7: CBX4 increases PHGDH transcription via recruiting GCN5 to the PHGDH promoter.
From: CBX4 plays a bidirectional role in transcriptional regulation and lung adenocarcinoma progression
A ChIP-qPCR assays were performed using anti-CBX4 or IgG, with primer pairs targeting the PHGDH promoter in A549 cells. B Genome browser view of the H2AK119ub signal on the PHGDH gene obtained by ChIP-seq in A549 cells. C-D Immunoprecipitation assays were carried out with anti-CBX4 (C) or anti-GCN5 (D), followed by immunoblotting analysis in A549 cells. ChIP-qPCR was performed using anti-GCN5 (E) and anti-H3K27ac (F) with primer pairs targeting the PHGDH promoter in A549 cells expressing control shRNA and shCBX4-1. For figures A, E, and F, each bar represents the mean ± SD for n = 3; *P < 0.05, **P < 0.01 versus control (Student’s t-test). G The sketch map presents the dual role of CBX4 in transcriptional regulation and LUAD progression.
