Fig. 5: Knockdown of BCKDK suppresses tumorigenesis.

a BCKDK knockdown-mediated inhibition in proliferation was rescued by G6PD. b Volcano of differentially expressed genes between shBCKDK and shCtrl BT-549 cells. c Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differential metabolites between shBCKDK and shCtrl cells of BT-549. d BCKDK overexpression-induced proliferation reversed by everolimus. e Changes in key proteins of the mTOR signalling pathway in differentially expressed BCKDK levels. f Changes in key proteins of the mTOR signalling pathway were analysed by western blot after BCKDK knockdown and G6PD overexpression. ***P < 0.001.