Fig. 2: FASN promotes filopodia formation in liver cancer cells by regulating FSCN1. | Cell Death & Disease

Fig. 2: FASN promotes filopodia formation in liver cancer cells by regulating FSCN1.

From: Deficiency of SIAH1 promotes the formation of filopodia by increasing the accumulation of FASN in liver cancer

Fig. 2

A Representative images and quantification of filopodia localization assay in HepG2 and Huh7 cells silencing FASN. Scale bar, 12.5 µm. B Representative images and quantification of filopodia localization assay in HepG2 and Huh7 cells overexpressing FASN. Scale bar, 12.5 µm. C, D Representative blots and quantification of FSCN1 expression in HepG2 and Huh7 cells silencing or overexpressing FASN. E Representative blots of CDC42, RAC1 and RHOA expression in HepG2 and Huh7 cells silencing or overexpressing FASN. F Quantification of cell invasion and migration while silencing or overexpressing FASN. G Representative images and quantification of filopodia localization assay of shControl, shFASN#2, and shFASN#2+Myc-FSCN1 groups in Huh7 cells. Scale bar, 12.5 µm. H Quantification of Huh7 cell invasion and migration. *P < 0.05, **P < 0.01, ***P < 0.001.

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