Fig. 4: CQ and BafA1, instead of 3-MA, protected ATC cells from PA-induced pyroptosis. | Cell Death & Disease

Fig. 4: CQ and BafA1, instead of 3-MA, protected ATC cells from PA-induced pyroptosis.

From: Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification

Fig. 4

A In 8505C and KHM-5M cells, PA (5 μM) is used in combination with 3-MA (3 mM, pre-treated for 2 h), BafA1 (100 nM, pre-treated for 2 h) and CQ (10 μM, pre-treated for 2 h) for 24 h, cell viability was measured by the CCK-8 assay. In 8505C and KHM-5M cells, PA (5 μM) is used in combination with BafA1 (100 nM, pre-treated for 2 h) and CQ (10 μM, pre-treated for 2 h) for 24 h, B morphologies were captured by microscope (scale bar: 20 μm), C relative LDH activity in culture mediums detected by the LDH assay kit. D After PA (0, 4, 5 and 6 μM) treatment for 24 h, the GAPDH, LC3-I/II, and p62 protein levels were measured by western blot. E, F In 8505C and KHM-5M cells, PA (5 μM) is used in combination with BafA1 (100 nM, pre-treated for 2 h) and CQ (10 μM, pre-treated for 2 h) for 24 h, and the GAPDH, LC3-I/II, and p62 protein levels were measured by western blot. Data are shown as mean ± SD for n = 3 (biological replicates). *p < 0.05, ***p < 0.001, ns, p > 0.05.

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