Fig. 4: LINC01637 binds to STAT6 protein and regulates STAT6 expression.

A, E Relative enrichment of LINC01637 mRNA associated with STAT6 protein was identified by RIP assays. The IgG group was a negative control to preclude nonspecific binding; (B, F) Agarose gel electrophoresis results by using RT-qPCR products from RIP assays; (C, G) The levels of LINC01637 and STAT6 mRNA were measured in UM cells after suppressing LINC01637; (D, H) The levels of LINC01637 and STAT6 mRNA were measured in UM cells that had an increased expression of LINC01637; (I, K) STAT6 in UM cells was analyzed by Western blotting, specifically focusing on the cells with LINC01637 knockdown; (J, L) Results from a Western blotting study of STAT6 in LINC01637-overexpressing UM cells. (Data were presented as the mean ± SD; n = 3; *p < 0.05, **p < 0.01, ***p < 0.001).