Fig. 6: USP25 attenuates the proteasomal degradation of phospho-STAT3. | Cell Death & Disease

Fig. 6: USP25 attenuates the proteasomal degradation of phospho-STAT3.

From: Ubiquitin-specific protease 25 ameliorates ulcerative colitis by regulating the degradation of phosphor-STAT3

Fig. 6

A Immunofluorescence was performed to examine the expression of p-STAT3 in NCM460 cells with or without USP25 low expression. Western blotting was performed to examine the indicated proteins in control groups vs. USP25 low-expressing NCM460 cells (B), colon tissues of Usp25−/− mice (C), USP25 overexpressing NCM460 cells (D), and USP25 overexpressing mice (E), with or without LPS/DSS treatment. F Co-immunoprecipitation assays showing the interaction between STAT3 and USP25 in 293T cells transfected with the indicated plasmids, with or without lambda protein phosphatase treatment. G Protein ubiquitination assays were performed in 293T cells transfected with the indicated plasmids, with or without static treatment. H Co-immunoprecipitation assays showing the interaction of USP25 with HA-STAT3-WT and mutation HA-STAT3-S727A/S521A/Y705F/T516A in 293T cells transfected with the indicated plasmids. I Ubiquitination assays to determine the deubiquitination of WT and mutated STAT3-S727A/S521A/Y705F/T516A in response to USP25 overexpression in 293T cells transfected with the indicated plasmids.

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